What is Hot stage microscopy?

Hot stage microscopy is a powerful method which is widely used to visually examine all kinds of thermal transitions. In the HS82, samples are heated or cooled while they are observed under the microscope. The HS84 DSC hot-stage can even simultaneously measure heat flow.

What is a hot stage microscope used for?

Hot stage microscopy (HSM) is the coupling of thermal analysis with microscopy for the solid-state characterization of materials as a function of temperature and time.

Why light microscope is referred to as optical microscope?

The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Polarised light may be used to determine crystal orientation of metallic objects.

What is the principle of dark field microscopy?

Principle. To view a specimen in dark field, an opaque disc is placed underneath the condenser lens, so that only light that is scattered by objects on the slide can reach the eye (figure 2). Instead of coming up through the specimen, the light is reflected by particles on the slide.

What are the general principles of microscopy?

To use the microscope efficiently and with minimal frustration, you should understand the basic principles of microscopy: magnification, resolution, numerical aperture, illumination, and focusing.

What is the basic principle of light microscope?

Principles. The light microscope is an instrument for visualizing fine detail of an object. It does this by creating a magnified image through the use of a series of glass lenses, which first focus a beam of light onto or through an object, and convex objective lenses to enlarge the image formed.

Which is the best description of hot stage microscopy?

Hot stage microscopy (HSM) is a thermal analysis technique that combines the best properties of thermal analysis and microscopy. HSM is rapidly gaining interest in pharmaceuticals as well as in other fields as a regular characterization technique.

How is HSM used in the medical field?

HSM combines traditional thermo-analytical techniques with latest technological advancements in imaging such as optical microscope, digital camera as well as the use of sophisticated software for analysis of the changes in the sample through the images generated during a thermal experiment.

When to use PLM and hot stage microscopy?

Using PLM and a hot stage allows for the detection of changes in polarization during heating, melting point, sublimation, phase transformation, crystallization, desolvation, decomposition, and purity.

Which is the most sensitive hot stage sensor?

With 56 thermal elements, the robust FRS 5 ceramic sensor in the HS84 is highly sensitive and has unprecedented temperature resolution. It is therefore ideally suited to all possible applications of the DSC hot-stage.