What is an organotypic model?

Organotypic models are 3D in vitro representations of an in vivo environment. Their complexity can range from an epidermal replica to the establishment of a cancer microenvironment. These models have been used for many years, in an attempt to mimic the structure and function of cells and tissues found inside the body.

What is organotypic cell culture?

Organotypic culture is defined as the culture of an organ collected from an organism. It is one method allowing the culture of complex tissues or organs. It allows the preservation of the architecture of the cultured organ and most of its cellular interactions.

What is a Histotypic culture?

Histotypic culture is defined as three-dimensional culture of one cell type, while the term organotypic implies the interaction of two or more cell types from a complex tissue or organ.

Is organ culture 3D culture?

Remarkable advances in three-dimensional (3D) cell cultures and organ-on-a-chip technologies have opened the door to recapitulate complex aspects of human physiology, pathology, and drug responses in vitro.

What are organotypic slices?

Organotypic brain slices are prepared from whole postnatal or adult brains (A), and 100- to 400-μm-thick sections are cut with a vibratome (B) and placed in an insert with 0.4-μm semipermeable pores (C). Brain slices display a strong vascular network, as seen by collagen IV staining (Alexa-488, green) (G).

What is meant by cell culture?

Listen to pronunciation. (sel KUL-cher) The growth of microorganisms such as bacteria and yeast, or human, plant, or animal cells in the laboratory. Cell cultures may be used to diagnose infections, to test new drugs, and in research.

What is meant by organ culture?

organ culture is the maintenance or growth in vitro of organ primordia or all or part of an organ in a way that may allow differentiation and preservation of the architecture and/or function.

What is a monolayer culture?

monolayer culture A type of culture in which cells are grown in a single layer on a flask or Petri dish containing the culture medium.

Why 3D culture is better than 2D?

The reason for the growth in 3D cell culture is it’s simply a better way of representing human tissue outside the body. 2D cell cultures only exist in two dimensions. More relevant cell models — Much better biomimetic tissue models make 3D cell cultures more physiologically relevant and predictive than 2D cultures.

What can slice cultures be used for?

Slice Cultures Acute slices are typically used for short-term electrophysiology experiments, while organotypic cultures are used to observe structural and morphological changes such as neuronal migration, axon outgrowth, or synapse formation.