By What is a 96-well format?
96-Well Format With Transparent Bottom The 96-well format microplate is available with the transparent bottom. Available in three different surface types including, non-treated, immunological and cell culture surfaces. They are manufactured out of white or black polystyrene (PS), with a transparent bottom made of (PS).
How many cells are in a 96-well plate?
Useful information for various sizes of cell culture dishes and flasks
| Catalog No. | Cells at confluency* | |
|---|---|---|
| Dishes | ||
| 24-well | 142475 | 0.24 x 106 |
| 48-well | 150687 | 0.12 x 106 |
| 96-well | 167008 | 0.04 x 106 |
How much volume does a 96-well plate have?
CulturPlate Microplates
| Well format | 24-well | 96-well |
|---|---|---|
| Well volume | 2.39 mL | 400 µL |
| Recommended working volume | 0.5-2.39 mL | 80 µL-350 µL |
| Height (mm) | 18.70 | 14.60 |
| Length (mm) | 127.80 | 127.76 |
What is the surface area of a 96-well plate?
0.32cm2
BD Falcon Tissue Culture Plates, 96-well; Well Shape: Flat; Well Volume: 0.37mL; Surface Area/Well: 0.32cm2.
What is a 96 well plate used for?
Industry standard, 96-well plates are designed for all common instruments and can be used for applications such as sample collection, compound preparation, combinatorial chemistry, high throughput screening, nucleic acid purification, bacterial culture growth, and plate replication.
How do you clean suspension cells in a 96 well plate?
Wash the cells by pipetting 200 μl ice-cold PBS into each well. Remove the PBS from the bottom of the wells by gentle aspiration. Repeat two times for a total of three washings. Pipette 30 μl protease-inhibitor-supplemented Cell Extraction Buffer into each well.
How do you seed a cell?
Take individual cell counts of all boxes, add them up and average them. Multiply the average with your dilution factor (in this case 10). This is the amount of cells in million per mL of your culture.
What is a 96 well microplate?
96-well microplates are used for antibiotic screens, cell-based assays and screening compounds. The flat bottom shape is ideal for microscopic and optical measurements.