By Which esterase is specific and which cell type does it stain?
Normal bone marrow cells Monocytes stain positively for alpha-naphthyl acetate esterase (nonspecific esterase) and alpha-naphthyl butyrate esterase but are alpha-naphthol AS-D chloroacetate-esterase-negative.
What does specific esterase stain?
The specific esterase is naphthol AS-D chloroacetate esterase (CAE). These identify the cells of the granulocytic series. It does not stain lymphocytes and monocytes. It can even be used to identify granulocytes in formalin fixed tissues. The cells of granulocytic series are negative with this stain.
What is Alpha naphthyl?
α-Naphthyl acetate method (Gomori 1950) This method employs a naphthyl acetate as the substrate; the enzyme releases a naphthol during the hydrolysis of the substrate. The α-naphthol is then coupled with a suitable diazonium salt to produce an insoluble azo dye at the site of enzyme activity.
What is non specific esterase?
Nonspecific esterase (NSE) is used to identify normal and leukemic mononuclear phagocytes cytochemically. Thus, the esterase isozymes represent two families of glycoproteins, both of them probable cell surface enzymes, resembling classical liver carboxyl or B-esterases (EC 3.1. 1 1).
Which type of anemia is usually present in a patient with acute leukemia?
Chemotherapy, radiation, and some drugs doctors use to treat leukemia may cause aplastic anemia. This is because some cancer therapies prevent bone marrow from making new, healthy blood cells. White blood cell counts drop first, then platelet counts, and finally, red blood cell counts.
What does Sudan black bind to?
Sudan Black B (SBB) is a lipophilic dye that binds irreversibly to an undefined granule component in granulocytes, eosinophils and some monocytes. It cannot be extracted from the stained granules by organic dye solvents and gives comparable information to that of MPO staining.
What cytochemical stain is best for differentiating AML from all?
Myeloperoxidase (MPO) MPO is useful for differentiating between ALL and AML blasts.
What are cytochemical stains used for?
Cytochemical stains are special stains used for staining peripheral blood and bone marrow smears that help in classifying and differentiating different types of leukaemias.
Is naphthol a phenol?
Naphthol, either of two colourless, crystalline organic compounds derived from naphthalene and belonging to the phenol family; each has the molecular formula C10H7OH.
What is chloroacetate esterase?
With the 91C kit, Naphthol AS-D Chloroacetate is enzymatically hydrolyzed by “specific esterase, ” liberating a free naphthol compound. This then couples with a diazonium compound, forming highly colored deposits at sites of enzyme activity. This enzyme is usually considered specific for cells of granulocytic lineage.
How do you demonstrate esterase?
Esterase activity is generally demonstrated by using α-naphthyl acetate or butyrate as substrates. The naphthol liberated by the enzyme is coupled with a diazonium salt to give an insoluble, brightly colored, azo dye.
What does an esterase do?
Esterases hydrolyze the compounds that contain ester, amide, and thioester bonds, which cause prodrug activation or detoxification. Among esterases, carboxylesterases are well known to be involved in the hydrolysis of a variety of drugs.