What is protein microarray technology?
A protein microarray (or protein chip) is a high-throughput method used to track the interactions and activities of proteins, and to determine their function, and determining function on a large scale. Its main advantage lies in the fact that large numbers of proteins can be tracked in parallel.
What are the different types of protein microarray?
Three types of protein microarrays are currently used to study the biochemical activities of proteins: analytical microarrays, functional microarrays, and reverse phase microarrays.
What is microarray analysis and its applications?
Microarray-based expression profiling allows us to identity families of genes as well as the important molecular and cellular events that might be important in complex processes like metastasis. Practical applications in future include diagnostic and prognostic management of patients.
What is the basic mechanism of functional protein microarray?
With regard to spotting proteins the two major mechanisms are contact and noncontact printing. Adapted from DNA microarray fabrication, the robotic contact printing tool is the most suitable for producing protein microarrays of high content due to the requirement to array large numbers of different proteins.
What is DNA microarray technology?
= Microarray technology is a developing technology used to study the expression of many genes at once. It involves placing thousands of gene sequences in known locations on a glass slide called a gene chip. A sample containing DNA or RNA is placed in contact with the gene chip.
What is the difference between DNA microarrays and protein microarrays?
The key difference between DNA and protein microarray is that DNA microarray is a collection of microscopic DNA spots attached to a solid surface like a glass slide, while protein microarray is an arrangement of purified proteins on a solid surface like a glass slide.
What is microarray technique?
Microarray technology is a developing technology used to study the expression of many genes at once. It involves placing thousands of gene sequences in known locations on a glass slide called a gene chip. A sample containing DNA or RNA is placed in contact with the gene chip.
What are the uses of DNA microarray?
The DNA microarray is a tool used to determine whether the DNA from a particular individual contains a mutation in genes like BRCA1 and BRCA2. The chip consists of a small glass plate encased in plastic. Some companies manufacture microarrays using methods similar to those used to make computer microchips.
What are the applications of DNA microarray?
Several applications of microarrays make use of SNP detection, including genotyping, forensic analysis, measuring predisposition to disease, identifying drug-candidates, evaluating germline mutations in individuals or somatic mutations in cancers, assessing loss of heterozygosity, or genetic linkage analysis.
How is DNA microarray used?
A microarray is a laboratory tool used to detect the expression of thousands of genes at the same time. DNA microarrays are microscope slides that are printed with thousands of tiny spots in defined positions, with each spot containing a known DNA sequence or gene.
What is microarray technology used for?
Why is DNA microarray an important tool?
DNA microarray is an effective tool in transcriptomics that helps us in studying and analyzing the mRNA expression of almost every gene present in an organism. With the availability of whole-genome sequencing of microorganisms, it has now become possible to identify the genes with potential for bioremediation.
How are DNA microarrays work?
DNA microarrays consist of an arrayed series of thousands of microscopic spots of DNA oligonucleotides, each containing specific DNA sequences, called ‘probes’ or ‘reporters’. Genes or DNA fragments of interest are amplified with PCR, cleaned up, and printed as a two-dimensional (2D) array using robotic microarraying devices that are able to dispense small quantities of solution at a precise location on a solid support, typically a glass slide due to its low inherent fluorescence.
What does microarray test for?
Microarray is a high resolution test to assess very small gains and losses (copy number variants) of genomic information and areas of homozygosity (which might suggest an autosomal recessive condition).
What is a cDNA microarray?
Chips are prepared by using cDNA.