Is Jaffe reaction specific for creatinine?

The colour produced from the sample is then compared in a colorimeter at wavelength of 505 nm with that produced by a known amount of creatinine under the same condition. The Jaffe reaction is known to be nonspecific when applied to the measurement of creatinine in plasma.

What type of reaction is the Jaffe reaction for creatinine?

PRINCIPLE. Creatinine reacts with picric acid in an alkaline solution to form a reddish colored complex. The reaction is commonly known as the Jaffe reaction and the red colored product as the Janovski complex.

Which method is used for estimation of creatinine?

Jaffe method
Still, creatinine remains the gold standard for measuring the glomerular filtration rate and kidney functioning. And Jaffe method remains the default method for estimation of creatinine.

Why Jaffe’s method is non specific for creatinine?

The Jaffe reaction is not specific for creatinine, the same reaction resulting in Jaffe-like chromogens show many interfering substances (ascorbic acid, bilirubin, proteins, ketones, cephalosporins and other drugs). Chemical and enzymatic methods show similar accuracy and day-to-day precision.

What is creatinine urine by Jaffe method?

Creatinine in serum or urine is determined by Jaffe’s reaction where creatinine produces quantitatively an orange color with picric acid in alkaline medium. After allowing an incubation time of 15 min at room temperature for color development the color is measured at 520 nm.

What is the Jaffe test?

The Jaffe reaction is a colorimetric method used in clinical chemistry to determine creatinine levels in blood and urine.

What is Jaffe method?

Why is serum creatinine measured?

The measure of serum creatinine may also be used to estimate how quickly the kidneys filter blood (glomerular filtration rate). Because of variability in serum creatinine from one person to another, the GFR may provide a more accurate reading on kidney function.

Which method estimates blood creatinine level most accurately?

Enzymatic creatinine assay. Widely accepted as one of the most accurate routine methods available at present and data in Table 3 indicates that this method produces results for patient samples that agree closely with IDMS.

What are the limitations of Jaffe’s test?

Conclusion. The Jaffe method is subject to bias due to interfering substances (loss of analytical specificity). The risk of misclassification is greatest at the 60 ml/min/1.73m2 decision limit; however, the risk of misclassification due to bias is much less than the risk of misclassification due to biological variation …

How Jaffe’s method is used to predicts serum blood creatinine level?

The Jaffe method is based on an alkaline picrate colorimetric reaction that tends to falsely elevate the true creatinine by up to 20% (for creatinine ~1 mg/dL) due to the presence of interfering, noncreatinine chromagens. This effect is greatest at the lower levels of creatinine typically observed in children.

What is creatinine normal value?

The typical range for serum creatinine is: For adult men, 0.74 to 1.35 mg/dL (65.4 to 119.3 micromoles/L) For adult women, 0.59 to 1.04 mg/dL (52.2 to 91.9 micromoles/L)

When to use the Folin-Wu blood sugar method?

Such a method has become particularly desirable in connection with the insulin treatment of infants, small children and others, from whom the usual amount of blood cannot be readily taken. It would be again of value in studying the blood sugar curves of adults under insulin treatment from whom blood must be taken at frequent intervals.

How is the colour of creatinine measured in colorimeter?

The method is based on the Jaffe reaction. Creatinine reacts with picrate ion formed in alkaline medium to develop a red-orange colour. The colour produced from the sample is then compared in a colorimeter at wavelength of 505 nm with that produced by a known amount of creatinine under the same condition.

How to prepare a protein free filtrate test?

Procedure of folin wu method for protein free filtrate preparation is quite easy and much like other tests. There are are few steps in this test that are given below: First of all take 2ml of blood. Then shift this blood to a container containing potassium oxalate. Then dilute the blood with distilled water. Add 14ml of Dist water.

How is optical density of glucose determined by Folin-UW method?

Principle: Glucose reduces the cupric ions present in the alkaline copper reagent to cuprous ions or the cupric sulfate is converted into cuprous oxide, which reduces the phosphomolybdic acid to phosphomolybdous acid, which is blue when optical density is measured at 420 nm.